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A high-level comparison of two of the most promising proteomic technologies — mass spectrometry and the SOMAscan assay
The SOMAscan® Assay: An Unprecedented Protein Biomarker Discovery and Development Tool
Dissecting complex biology requires a proteomic technology that can measure both broadly (thousands of proteins simultaneously) and deeply (across a wide range of concentrations).
Only the SOMAscan assay can do both, thanks to the distinctive properties of the SOMAmer reagents used in the assay. In essence, the SOMAscan assay overcomes a difficult protein measurement challenge by changing it into a straightforward DNA measurement solution.
Figure: Highlights of the SOMAscan assay.
- SOMAmer reagents attached to streptavidin beads bind to proteins in the sample mix (i)
- The proteins that are bound to their specific SOMAmer reagents are then biotinylated (ii)
- The SOMAmer-protein complexes are released by photocleaving the linker, and non-specific SOMAmer-protein complexes are separated (center pair) (iii)
- Biotinylated proteins are bound to a second streptavidin bead (iv)
- Bound SOMAmer reagents are removed from their protein targets (v)
- SOMAmer reagents are collected and denatured (vi)
- SOMAmer reagents are measured using standard DNA analysis techniques like microarrays (vii).
More information can be found in the SOMAscan Assay Technical White Paper.