Are there preferred sources of target protein (e.g. recombinant systems, etc.)?
Can a custom SOMAmer reagent be designed to discriminate between protein variants or post- translational modifications (PTMs) of the target protein?
How many libraries are used to identify candidate SOMAmer reagents that bind to the target analyte?
How many SOMAmer binding reagents will be obtained during the custom SOMAmer Discovery Service process?
How much target protein is required to design a custom SOMAmer reagent?
What are acceptable fusion tags on the target analyte?
What are the expected dissociation constants (Kd) for the candidate SOMAmer binding reagents?
What buffers are compatible with the SOMAmer Discovery process (i.e. in what buffers can the target analyte be suspended)?
What is the minimum size protein target that can be used when designing a custom SOMAmer reagent?
The target protein should be a minimum of 25 amino acids excluding fusion tags (e.g. polyHis, IgG, biotin, etc.). If the target protein of interest is less than 25 amino acids, it may be possible to design a custom SOMAmer reagent, but please contact SomaLogic Technical Support at firstname.lastname@example.org or by telephone at 1-800-324-0783 or 303-625-9000.
Will the epitope on the target analyte be known?
Crystal structure analyses of a small number of SOMAmer reagent-protein complexes indicate SOMAmer reagents bind large, conformational epitopes, but the exact epitope of every SOMAmer reagent is not known.