The SOMAscan® assay: An unprecedented protein biomarker discovery and development Tool

Dissecting complex biology requires a proteomic technology that can measure both broadly (thousands of proteins simultaneously) and deeply (across a wide range of concentrations). Only the SOMAscan assay can do both, thanks to the distinctive properties of the SOMAmer reagents used in the assay. In essence, the SOMAscan assay overcomes a difficult protein measurement challenge by changing it into a straightforward DNA measurement solution.
Researchers can access the SOMAscan assay for their own investigations either as a service from SomaLogic directly, or by having the SOMAscan assay deployed at their own institution.

Figure: Highlights of the SOMAscan assay.

  • SOMAmer reagents attached to streptavidin beads bind to proteins in the sample mix (i)
  • The proteins that are bound to their specific SOMAmer reagents are then biotinylated (ii)
  • The SOMAmer-protein complexes are released by photocleaving the linker, and non-specific SOMAmer-protein complexes are separated (center pair) (iii)
  • Biotinylated proteins are bound to a second streptavidin bead (iv)
  • Bound SOMAmer reagents are removed from their protein targets (v)
  • SOMAmer reagents are collected and denatured (vi)
  • SOMAmer reagents are measured using standard DNA analysis techniques like microarrays (vii).

More information can be found in the SOMAscan Assay Technical White Paper.